Effect of human immunodeficiency virus-1 protease inhibitors on the clearance of human herpesvirus 8 from blood of human Immunodeficiency virus-1-infected patients

Abstract Background.—Human immunodeficiency virus 1 (HIV-1)–infected individuals are commonly infected with herpesviruses, including cytomegalovirus, herpes simplex virus, varicella-zoster virus, and human herpesvirus 8 (HHV-8, also known as Kaposi sarcoma–associated herpesvirus [KSHV]). Previous studies have demonstrated that coinfection with herpesviruses can modulate HIV-1 replication. This can occur either through direct interaction between the 2 viruses or through secondary effects resulting from the release of cellular factors in response to infection. Objective.—To investigate HIV-1 replication in the presence and absence of HHV-8. Design and Methods.—HIV-1 replication was analyzed following culture of HIV-1–infected CD4+ T cells in the presence of HHV-8 infected B-cell lines or control, uninfected B-cell lines. To confirm and extend the results of these in vitro studies, HIV-1–infected T cells were injected into human skin transplanted onto severe combined immunodeficient mice. The human skin was also injected with purified HHV-8 or phosphate-buffered saline as a control and HIV replication measured in biopsy specimens taken 5 to 8 days later. Results and Conclusions.—The results demonstrated a significant increase in HIV-1 replication in the presence of HHV-8 in both the in vitro and in vivo model systems. Although the mechanism responsible for HHV-8 induction of HIV-1 replication remains to be identified, the results indicate that these 2 viruses may interact at the molecular level in coinfected patients, resulting in increased HIV-1 viral load.

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Searching for Human Herpesvirus 8 Antibodies in Serum Samples from Patients Infected with Human Immunodeficiency Virus Type 1 and Blood Donors from São Paulo, Brazil

The Journal of Infectious Diseases ◽

10.1086/314789 ◽

1999 ◽

Vol 179 (6) ◽

pp. 1591-1592 ◽

Cited By ~ 16

Author(s):

Adele Caterino‐de‐Araujo ◽

Maria Luisa Calabrò ◽

Elizabeth de los Santos‐Fortuna ◽

Jamal Suleiman ◽

Luigi Chieco‐Bianchi

Keyword(s):

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Blood Donors ◽

Human Herpesvirus ◽

Human Herpesvirus 8 ◽

Serum Samples ◽

Herpesvirus 8 ◽

Immunodeficiency Virus

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Association of Primary Pleural Effusion Lymphoma of T-Cell Origin and Human Herpesvirus 8 in a Human Immunodeficiency Virus–Seronegative Man

Archives of Pathology & Laboratory Medicine ◽

10.5858/2001-125-1246-aoppel ◽

2001 ◽

Vol 125 (9) ◽

pp. 1246-1248 ◽

Cited By ~ 1

Author(s):

Emmanuèle Lechapt-Zalcman ◽

Dominique Challine ◽

Marie-Hélène Delfau-Larue ◽

Corinne Haioun ◽

Dominique Desvaux ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

T Cell ◽

Dna Sequences ◽

Primary Effusion Lymphoma ◽

Polymerase Chain Reaction Amplification ◽

Human Herpesvirus ◽

Body Cavity ◽

Human Herpesvirus 8 ◽

Herpesvirus 8 ◽

Immunodeficiency Virus

Abstract We describe a case of an 87-year-old human immunodeficiency virus (HIV)–negative man who developed a primary pleural lymphoma without any identifiable tumor mass associated with human herpesvirus 8 (HHV-8) infection. A large T-cell lymphoma was diagnosed based on morphologic, immunophenotypic, and molecular findings. The HHV-8 DNA sequences were detected using specific polymerase chain reaction amplification in the lymphomatous effusion. Study of the patient's serum confirmed the HHV-8 infection. This case report displays the characteristic features of HHV-8–related body cavity-based lymphoma/primary effusion lymphoma previously reported in HIV-seronegative patients, except that it is of T-cell origin. Whether this case may be included or not within the primary effusion lymphoma entity, the association of a pleural T-cell non-Hodgkin lymphoma with HHV-8 infection raises the question of the possible occurrence of T cells as the target of malignant transformation associated with HHV-8 infection.

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